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▀ ▀▄▄ ▀▀▀▄▄ █▌ ▐▌ sCr! ▀▓▀ ▄█▄ ▀▀▀█▄▄▄▄▄▄
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▄▄ ▀█▄ ▀▄▄ ▀▀▀▀▀▀▀ It goes AGAiN, AGAiN... ▀▀▀▀▀ ▄██▀ ▀▄▄██▀
▄██▌ ▀▓▄ ██▄ ▄█▀ ▄█▀ ▄▄
▓▓███▄ ▐█▌███▌ GenePix Pro v6.0.1.26 ▄██ ▄▓▀ ▐██▄
▓▓▓████▄▄▄▓▀▄██▓▓ ▐███▐█▌ ▄███▓▓
▀██████▄▄▄█▀ █▓▀ (c) Molecular Devices ▓▓██▄▀▓▄▄▄████▓▓▓
▄▄▄▀▀████▀▀ ▄▀ ▀▓█ ▀█▄▄▄██████▀
███ ▄▄▀ Cracker...: AGAiN 2006 ▀▄ ▀▀████▀▀▄▄▄
███▄ ▄▄██▀ Supplier..: AGAiN 2006 ▀▄▄ ███
▀███▀███▀ Packager..: AGAiN 2006 ▀██▄▄ ▄███
▄ ▄██▀ Tester....: AGAiN 2006 ▓███▀███▀
▓ ███ ▓███
▒ ▐██▌ OS..........: Windows ▐▌███
░ ▐██▌ Language....: English █ ███
███ Protection..: Dongle █ ▐██▌
▄ ▀██▄ Size........: 05 x 4.77mb ▐▌ ███
▄▓▄ ▀▀▀ Date........: 17/06/2006 ▄▀ ███
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▀ ▄▄▀▀▀████▄ ▀██▄▄ ▄ ▄█▀ ▀▀ ▄▄▀ █▀▀█▄▄▀▀▀▀▀▀▀▀▀ ▄▀ ▄▄▄▀▀▀
▄██ ▀▀██▄▄ ▀▀████▄▄▄▄▄████████▀▀ ▄█ ▀▀█▄▄▄ ▄▄▄▀▀ ▄█▀
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▀▀████▄▄ ▐█▄▄▄▄▀▀▀ ▀▀▀▄▄▄▄ ▄▄▀
▄▀ ▀███▓▄ R e l e a s e I n f o ▀▀▀▀▀▀▄▄▄▄▄▄▄▄▄▄▀▀▀
█▓ █▄████▀ ▄▄▄ ▀▄
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▀█▄▄ GenePix Pro is the industry standard microarray image ▀▀▄▄██▀▀
▄▄▀▀██▄▄ analysis software because of its unique combination of ▄█▀▀
▄██ ▀██ imaging and analysis tools, visualizations, automation █▓ █████▀
▓██▄ ▄▀ capabilities, performance and ease of use. █▓ █▀████▄
▀▀████▄▄ ▀▄ ▄███▓▀
▄▀ ▀███▓▄ IMAGING TOOLS ▄▄████▀▀
█▓ █▄████▀ ▓██▀ ▀▄
█▓ █████▄ Display ▀██ ▄██
▀█▄▄ GenePix Pro displays each of up to four single ▀▀▄▄██▀▀
▄▄▀▀██▄▄ wavelength images in a different user-configurable ▄█▀▀
▄██ ▀██ color, then constructs color ratio images from any █▓ █████▀
▓██▄ ▄▀ three pairs of channels as specified by the user. █▓ █▀████▄
▀▀████▄▄ Four-channel imaging can be used to analyze multiple ▀▄ ▄███▓▀
▄▀ ▀███▓▄ channels from the same scan, or multiple one- or ▄▄████▀▀
█▓ █▄████▀ two-color images from different scans. Select colors ▓██▀ ▀▄
█▓ █████▄ from a fully customizable color palette. ▀██ ▄██
▀█▄▄ ▀▀▄▄██▀▀
▄▄▀▀██▄▄ Zooming buttons, hot keys, a Navigation Map and an ▄█▀▀
▄██ ▀██ efficient Windows implementation make it fast and easy █▓ █████▀
▓██▄ ▄▀ to move around large images. █▓ █▀████▄
▀▀████▄▄ ▀▄ ▄███▓▀
▄▀ ▀███▓▄ Feature-Finding ▄▄████▀▀
█▓ █▄████▀ GenePix Pro uses a set of proprietary feature-finding ▓██▀ ▀▄
█▓ █████▄ algorithms to find circular, square or irregular ▀██ ▄██
▀█▄▄ features. Every pixel in a region around the feature ▀▀▄▄██▀▀
▄▄▀▀██▄▄ is examined by a local alignment algorithm and ▄█▀▀
▄██ ▀██ assigned to feature or background. Global alignment █▓ █████▀
▓██▄ ▄▀ algorithms determine the translation, rotation and █▓ █▀████▄
▀▀████▄▄ skew of blocks of features. ▀▄ ▄███▓▀
▄▀ ▀███▓▄ ▄▄████▀▀
█▓ █▄████▀ Measuring Tools ▓██▀ ▀▄
█▓ █████▄ Use the Measuring Tools to make detailed calculations ▀██ ▄██
▀█▄▄ from specific regions on your arrays. GenePix Pro ▀▀▄▄██▀▀
▄▄▀▀██▄▄ automatically calculates statistics and graphs the ▄█▀▀
▄██ ▀██ pixel data in the drawn region. Draw lines, polylines, █▓ █████▀
▓██▄ ▄▀ and wide lines to generate an intensity profile graph █▓ █▀████▄
▀▀████▄▄ of the line. Draw rectangles, polygons, and ellipses ▀▄ ▄███▓▀
▄▀ ▀███▓▄ to generate a histogram of pixel intensities inside ▄▄████▀▀
█▓ █▄████▀ the region. Use these tools to obtain quick ▓██▀ ▀▄
█▓ █████▄ measurements and channel comparisons without gridding ▀██ ▄██
▀█▄▄ or analysis. ▀▀▄▄██▀▀
▄▄▀▀██▄▄ ▄█▀▀
▄██ ▀██ Image Alignment █▓ █████▀
▓██▄ ▄▀ If your non-Axon images have to be aligned before they █▓ █▀████▄
▀▀████▄▄ can be analyzed, you have a number of methods of ▀▄ ▄███▓▀
▄▀ ▀███▓▄ aligning them in GenePix Pro. Drag one image over ▄▄████▀▀
█▓ █▄████▀ another, or let GenePix Pro find the optimal alignment ▓██▀ ▀▄
█▓ █████▄ for you. ▀██ ▄██
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▄▄▀▀██▄▄ Barcode Reading ▄█▀▀
▄██ ▀██ GenePix Pro automatically reads barcodes from images. █▓ █████▀
▓██▄ ▄▀ Barcodes can be included automatically in file names, █▓ █▀████▄
▀▀████▄▄ and are exported with extracted data. ▀▄ ▄███▓▀
▄▀ ▀███▓▄ ▄▄████▀▀
█▓ █▄████▀ ANALYSIS TOOLS ▓██▀ ▀▄
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▀█▄▄ Background Subtraction ▀▀▄▄██▀▀
▄▄▀▀██▄▄ Choose your background subtraction method from a ▄█▀▀
▄██ ▀██ number of local and global methods, or correct your █▓ █████▀
▓██▄ ▄▀ data based on negative controls. Local background █▓ █▀████▄
▀▀████▄▄ correction is recommended for most microarrays because ▀▄ ▄███▓▀
▄▀ ▀███▓▄ even small variations in the background signal can ▄▄████▀▀
█▓ █▄████▀ affect ratio values. For arrays on which the spots are ▓██▀ ▀▄
█▓ █████▄ too close together to calculate an accurate local ▀██ ▄██
▀█▄▄ background, you can specify a single value to subtract ▀▀▄▄██▀▀
▄▄▀▀██▄▄ from all features, for each channel. If you have ▄█▀▀
▄██ ▀██ negative controls on your microarray, use the nearest █▓ █████▀
▓██▄ ▄▀ negative control, the mean of the nearest n negative █▓ █▀████▄
▀▀████▄▄ controls, or the mean of all negative controls as the ▀▄ ▄███▓▀
▄▀ ▀███▓▄ background value. At any time you can visually review ▄▄████▀▀
█▓ █▄████▀ the local background region assigned to a feature. ▓██▀ ▀▄
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▀█▄▄ The Feature Viewer ▀▀▄▄██▀▀
▄▄▀▀██▄▄ Use the Feature Viewer to get on-the-fly access to ▄█▀▀
▄██ ▀██ individual feature data, without analyzing the entire █▓ █████▀
▓██▄ ▄▀ image. The Feature Viewer displays images and data for █▓ █▀████▄
▀▀████▄▄ all acquisition wavelengths, and the three ▀▄ ▄███▓▀
▄▀ ▀███▓▄ user-specified ratios. Feature Indicators are drawn ▄▄████▀▀
█▓ █▄████▀ directly in the Feature Viewer to instantly evaluate ▓██▀ ▀▄
█▓ █████▄ individual features on a large array without having to ▀██ ▄██
▀█▄▄ zoom-in on each one. ▀▀▄▄██▀▀
▄▄▀▀██▄▄ ▄█▀▀
▄██ ▀██ The Feature Pixel Plot █▓ █████▀
▓██▄ ▄▀ The Feature Pixel Plot provides additional detailed █▓ █▀████▄
▀▀████▄▄ information about individual features, including a ▀▄ ▄███▓▀
▄▀ ▀███▓▄ scatter plot comparing individual pixels in the ▄▄████▀▀
█▓ █▄████▀ selected wavelength channels. ▓██▀ ▀▄
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▀█▄▄ Advanced Quality Control Flagging ▀▀▄▄██▀▀
▄▄▀▀██▄▄ General Boolean queries allow you to flag or highlight ▄█▀▀
▄██ ▀██ data based on user-defined criteria. Complex criteria █▓ █████▀
▓██▄ ▄▀ can be defined based on any measurements from the █▓ █▀████▄
▀▀████▄▄ Results table. Features identified by the query can be ▀▄ ▄███▓▀
▄▀ ▀███▓▄ marked with quality flags ('good', 'bad'), highlighted ▄▄████▀▀
█▓ █▄████▀ for further evaluation, or used to calculate a ▓██▀ ▀▄
█▓ █████▄ normalization factor. Queries can be saved for use on ▀██ ▄██
▀█▄▄ other arrays. Speed through analysis by defining in ▀▀▄▄██▀▀
▄▄▀▀██▄▄ advance the criteria you need to identify and isolate ▄█▀▀
▄██ ▀██ both good and poor data, or control features. █▓ █████▀
▓██▄ ▄▀ █▓ █▀████▄
▀▀████▄▄ Find ▀▄ ▄███▓▀
▄▀ ▀███▓▄ Use the Find function to search for all features with ▄▄████▀▀
█▓ █▄████▀ a given Name or ID from anywhere in the GenePix Pro ▓██▀ ▀▄
█▓ █████▄ interface. ▀██ ▄██
▀█▄▄ ▀▀▄▄██▀▀
▄▄▀▀██▄▄ Extracting Data from Images ▄█▀▀
▄██ ▀██ A single click of the Analyze button automatically █▓ █████▀
▓██▄ ▄▀ calculates up to 108 different measurements for each █▓ █▀████▄
▀▀████▄▄ feature and displays the data on the in a spreadsheet. ▀▄ ▄███▓▀
▄▀ ▀███▓▄ ▄▄████▀▀
█▓ █▄████▀ Normalization ▓██▀ ▀▄
█▓ █████▄ Normalize data with linear, ratio-based methods, on ▀██ ▄██
▀█▄▄ normalization features, selected features or all ▀▀▄▄██▀▀
▄▄▀▀██▄▄ features. For advanced users, GenePix Pro provides ▄█▀▀
▄██ ▀██ fully general Boolean searches to define normalization █▓ █████▀
▓██▄ ▄▀ features. █▓ █▀████▄
▀▀████▄▄ ▀▄ ▄███▓▀
▄▀ ▀███▓▄ Web Integration ▄▄████▀▀
█▓ █▄████▀ GenePix Pro is fully integrated with web-accessible ▓██▀ ▀▄
█▓ █████▄ databases. No longer is your data isolated, waiting to ▀██ ▄██
▀█▄▄ be imported into a larger database or analysis ▀▀▄▄██▀▀
▄▄▀▀██▄▄ program. Create a list of your favorite URLs to choose ▄█▀▀
▄██ ▀██ from, or store a preferred URL in each GenePix Array █▓ █████▀
▓██▄ ▄▀ List file. Point at a feature, select Go To Web, and █▓ █▀████▄
▀▀████▄▄ your default web browser opens the database and ▀▄ ▄███▓▀
▄▀ ▀███▓▄ automatically queries the database for information ▄▄████▀▀
█▓ █▄████▀ about the selected substance name or ID. ▓██▀ ▀▄
█▓ █████▄ ▀██ ▄██
▀█▄▄ As with all other GenePix Pro features, Go To Web is ▀▀▄▄██▀▀
▄▄▀▀██▄▄ fully scriptable, so you can construct a custom view ▄█▀▀
▄██ ▀██ of the data in a Report and embed hyperlinks to your █▓ █████▀
▓██▄ ▄▀ favorite databases. █▓ █▀████▄
▀▀████▄▄ ▀▄ ▄███▓▀
▄▀ ▀███▓▄ Automation ▄▄████▀▀
█▓ █▄████▀ Using a simple series of checkboxes, you can specify ▓██▀ ▀▄
█▓ █████▄ automation for any or all steps in the GenePix system ▀██ ▄██
▀█▄▄ from scanning through analysis and file saving. ▀▀▄▄██▀▀
▄▄▀▀██▄▄ Scripting allows even greater flexibility for ▄█▀▀
▄██ ▀██ customized automation. █▓ █████▀
▓██▄ ▄▀ █▓ █▀████▄
▀▀████▄▄ Standard Data Formats ▀▄ ▄███▓▀
▄▀ ▀███▓▄ Axon Instruments' GAL and GPR file formats are ▄▄████▀▀
█▓ █▄████▀ standards in the microarray industry. ▓██▀ ▀▄
█▓ █████▄ ▀██ ▄██
▀█▄▄ GenePix Array List (GAL) Files ▀▀▄▄██▀▀
▄▄▀▀██▄▄ Easily construct lists describing the position and ▄█▀▀
▄██ ▀██ content of each spot on an array from plain text █▓ █████▀
▓██▄ ▄▀ files. Substance name and ID lists can be pasted █▓ █▀████▄
▀▀████▄▄ directly into the array settings file to create a ▀▄ ▄███▓▀
▄▀ ▀███▓▄ GenePix Array List (GAL) file. GAL files can also be ▄▄████▀▀
█▓ █▄████▀ created from a collection of microwell plate files ▓██▀ ▀▄
█▓ █████▄ using the GenePix Array List Generator. Add as many ▀██ ▄██
▀█▄▄ columns as you like to the GAL file to track any ▀▀▄▄██▀▀
▄▄▀▀██▄▄ sample information along with GenePix results. See the ▄█▀▀
▄██ ▀██ GenePix File Formats page for instructions. █▓ █████▀
▓██▄ ▄▀ █▓ █▀████▄
▀▀████▄▄ GenePix Results (GPR) Files ▀▄ ▄███▓▀
▄▀ ▀███▓▄ GenePix Pro analysis Results are displayed in their ▄▄████▀▀
█▓ █▄████▀ own spreadsheet. Quality and normalization flags ▓██▀ ▀▄
█▓ █████▄ appear graphically in their own column for easy ▀██ ▄██
▀█▄▄ identification. Sort, flag, review images, save or ▀▀▄▄██▀▀
▄▄▀▀██▄▄ export all from the Results tab. Save data directly to ▄█▀▀
▄██ ▀██ Acuity microarray informatics software. █▓ █████▀
▓██▄ ▄▀ █▓ █▀████▄
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▄▀ ▀▀▀▀▀▀▄▄▄▄▄▄ ▀▀▀▀▀▄▄ ▄▄█▀▀▀█▄▄ ▄▄▄ █▓ ▀ ▀▀▄
█▓ ▀▀▀▀▀▄▄ ▀▄ █████▓▓▓▓▓█ ▄██▀ ▄█▀ ▀▄ ▐▌
█▓ I n s t a l l I n f o █ ▄▀ ███████▓▓▓█ ███ █▀█ ▄▄▀ ▀▀▄▄▄▄▀
▀█▄▄ ▄▄█▀▀ ▀▀█████▀▀ ▀██▄▄ ▄▄▀ ▀▀▀▀ ▄▀
▄▄▀▀██▄▄ █▓ █████▀
▄██ ▀██ Unzip and unrar release, install it. xtract the █▓ █▀████▄
▓██▄ ▄▀ included patch into your app''s dir and run it. ▀▄ ▄███▓▀
▀▀████▄▄ ▄▄████▀▀
▄▀ ▀███▓▄ Enjoy. ▓██▀ ▀▄
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▀█▄▄ ▀▄▄ ▄▀ ▀▀▄ ▀▄▀▀▄▄ ▄▄██▄▄ ▄▄ ▄▄▄██▓▓▓▓▄▄▄ ▄█▀▀
▄▄▀▀██▄▄ ▀▀▀ ▄▀ █ ▀▀▄ ▀ ▀█▄ ▀▓▓▀ ▀ ▀▀▄ █▓ █████▀
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▓██▄ ▄▀ ▄▄▀ ▄█▀ - G r o u p ! - ▀▄ ▄███▓▀
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█▓ █▄████▀ There's the phoenix, immortality born with the flames, ▀██ ▄██
█▓ █████▄ strength rised from the ashes, mythical magnificence ▀▀▄▄██▀▀
▀█▄▄ resurrected from the sky - soul of the spiritual ▄█▀▀
▄▄▀▀██▄▄ being, intense excitement, cosmic fire the world began █▓ █████▀
▄██ ▀██ and will end in, death and rebirth regenerated. █▓ █▀████▄
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▀▀████▄▄ Things fade. Things die. He has been born again, he ▄▄████▀▀
▄▀ ▀███▓▄ lives again. WE LIVE AGAiN. Deathless inspiration. ▓██▀ ▀▄
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█▓ █████▄ [email protected] ▀▀▄▄██▀▀
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▄▀ ▀███▓▄ G r e e t i n g s ▓██▀ ▀▄
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█▓ █████▄ SSG - LND - SAC ▀▀▄▄██▀▀
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▄▄▄▄▄▄ ▄▄▀▀ ▄▄▄▄ sCr/strick9! ▀▀▀ ▀▀▀██████▄▄▄
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and layout by Scour/SAC 2003. ▀██▄ ▀▀▀██████▄███▀
Ascii footer by Scour and Strick9. ▄ ▄██▀ ▀▀▀▀ ▄▓▄
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AGAiNST THE ODDS! ▀█▄
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